| DOI | Resolve DOI: https://doi.org/10.1021/np200689w |
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| Author | Search for: Dupuis, S.N.; Search for: Veinot, T.; Search for: Monro, S.M.A.; Search for: Douglas, S.E.1; Search for: Syvitski, R.T.1; Search for: Goralski, K.B.; Search for: McFarland, S.A.; Search for: Jakeman, D.L. |
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| Affiliation | - National Research Council of Canada. NRC Institute for Marine Biosciences
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| Format | Text, Article |
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| Subject | angucycline derivative; antineoplastic agent; Jadomycin dextro norleucine; Jadomycin dextro norvaline; natural product; norleucine; norvaline; unclassified drug; amino acid substitution; animal cell; antibacterial activity; bacterium culture; cancer cell culture; cancer inhibition; cell density; concentration response; cytotoxicity; DNA cleavage; drug isolation; Gram negative bacterium; Gram positive bacterium; human cell; in vitro study; LC 50; minimum inhibitory concentration; Staphylococcus epidermidis; Streptomyces venezuelae; Amino Acid Sequence; Amino Acids; Chloramphenicol; DNA; Drug Screening Assays, Antitumor; Gram-Negative Bacteria; Gram-Positive Bacteria; Isoquinolines; Microbial Sensitivity Tests; Molecular Structure; Norleucine; Oxazolone; Streptomyces; Valine |
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| Abstract | Streptomyces venezuelae ISP5230 is recognized for the production of chloramphenicol and the jadomycin family of natural products. The jadomycins are angucycline natural products containing a unique oxazolone ring incorporating an amino acid present in the minimal culture media. Substitution of different amino acids results in products of varying biological activity. Analysis of cultures of S. venezuelae ISP5230 incubated with l- and d-norvaline and l- and d-norleucine indicated that only the d-configured amino acids were incorporated into the natural products. Subsequently, jadomycin DNV and jadomycin DNL were isolated and characterized (titers 4 and 9 mg L -1, respectively). The compounds were evaluated in the National Cancer Institute cell line cancer growth inhibition and cytotoxicity screens, for antimicrobial activity against selected Gram-positive and Gram-negative bacteria, and as DNA-cleavage agents in vitro. © Published 2011 by the American Chemical Society. |
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| Publication date | 2011 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21271103 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 01fa4f36-30fd-4c00-a917-cfaba38d9ec8 |
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| Record created | 2014-03-24 |
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| Record modified | 2020-04-21 |
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