Characterization and nutritional properties of Methanothrix concilii sp. nov., a mesophilic, aceticlastic methanogen

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1139/m84-221
Author	Search for: Patel, G. B.¹
Affiliation	National Research Council of Canada. NRC Institute for Biological Sciences
Format	Text, Article
Abstract	A nonmotile, rod-shaped, nonspore-forming, mesophilic methanogenic bacterium was isolated from sewage sludge. The cells stained Gram negative. In the presence of CO2, the isolate was able to grow and produce significant amounts of methane from acetic acid. No growth or methane formation was observed when H2, methanol, pyruvate, propionate, butyrate, formate, or trimethylamine were provided as substrates in the presence of CO2. About 0.95 mol of CH4 was produced per mole of acetic acid consumed. The optimum pH and temperature for growth were 7.1–7.5 and 35–40 °C, respectively. The mass doubling time was about 24 h under optimum growth conditions. The almost complete inhibition of methane formation by 10 μM 2-bromoethanesulfonic acid (2-BES) was reversed in the presence of 50 μM 2-mercaptoethanesulfonic acid (HS-CoM). D-Cycloserine at 0.1 mg/mL concentration caused complete inhibition of growth. Sludge fluid enhanced the rate of methane formation, whereas 0.5% (w/v) yeast extract was inhibitory. The optimum initial CO2 (added as NaHCO3) for growth on acetic acid was 1.8 mM. The DNA base composition was 61.25 ± 0.60 mol% G + C. On the basis of its characteristics, this isolate is classified as Methanothrix concilii sp. nov. and the type strain is GP6 (=NRC 2989).
Publication date	1984
In	Canadian Journal of Microbiology 30, no. 11 (1984): 1383–1396.
Language	English
NRC number	PATEL1984 NRCC 23743
NPARC number	9381501
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Record identifier	027596b9-6033-42cf-a86f-708856a38766
Record created	2009-07-10
Record modified	2020-03-13

Date modified:: 2025-11-11