| DOI | Resolve DOI: https://doi.org/10.1016/S0924-2031(01)00161-8 |
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| Author | Search for: McCrae, Kimberly C.; Search for: Mantsch, Henry H.1; Search for: Thliveris, James A.; Search for: Scott, J. Elliott; Search for: Shaw, R. Anthony1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biodiagnostics
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| Format | Text, Article |
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| Conference | Shedding New Light On Disease: Optical Diagnostics for the New Millennium, June 25-30, 2000, Winnipeg, Canada |
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| Subject | Lung neoplasm; Type II cells; Electron microscopy; Infrared microspectroscopy; Lung classification; Genetic algorithm |
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| Abstract | Methods based on infrared microspectroscopy were explored as a means to distinguish normal from neoplastic lung tissue. Mice were exposed to urethane, a known environmental carcinogen. After 3–8 months, lungs were removed, snap-frozen, sectioned and analyzed by standard histological methods and by infrared microspectroscopy. Neoplasms were readily observed in mice treated with urethane. Ultra-structurally, the neoplasms were composed entirely of type II alveolar cells displaying intracellular lamellar bodies. A fusion of two spectral pre-processing techniques, optimal region selection and linear discriminant analysis (LDA), was used to search for infrared spectral signatures distinguishing normal from neoplastic tissue. These techniques showed clear and reproducible differences between the complex spectra of these tissue types, suggesting that infrared microspectroscopy in conjunction with spectral processing technology may be useful to reveal subtle spectral differences occurring following induction of neoplastic changes and to interpret their biochemical origins. |
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| Publication date | 2002-02-28 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | 1936 |
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| NPARC number | 9722783 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 0353c8cf-2bc3-40c9-b81f-f20a64fc144f |
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| Record created | 2009-07-17 |
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| Record modified | 2020-04-06 |
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