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Sulphur requirements for growth of Acetivibrio cellulolyticus

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1139/m82-117
AuthorSearch for: 1; Search for: 1; Search for: 2
Affiliation
  1. National Research Council of Canada
  2. National Research Council of Canada. NRC Institute for Biological Sciences
FormatText, Article
Abstract
The sulphur requirement for growth of Acetivibrio cellulolyticus was determined using a mineral salts basal medium reduced with titanium(III) citrate and supplemented with 1% (w/v) cellobiose and 0.05% (w/v) yeast extract. Inorganic sulphide (Na2Sāˆ™9H2O) in the range of 0.7–1.0 mM was found to support maximum growth whereas 8.0 mM sulphide caused complete inhibition. Of the other sulphur-containing compounds tested (cystine, sodium thioglycollate, methionine, glutathione, homocystine, S-methyl-L-cysteine, inorganic sulphate) cysteine-HCl at 10–15 mM concentration was the only one which supported maximum growth that was at least 80% of that obtained in the optimum sulphide medium. A modified medium incorporating the optimum inorganic sulphide concentration supported maximum growth that was about twice that obtained in a conventional medium reduced with cysteine-Na2S. The possible reasons for the inhibition of growth in defined media containing Na2S as the sole reducing agent and the sulphur source are discussed.
Publication date
In
  • Canadian Journal of Microbiology 28 (1982): 772ā€“777.
LanguageEnglish
NRC numberPATEL1982A
NRCC 20199
NPARC number9372042
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Record identifier03daa648-e011-43b8-ad6b-13bc685a9b55
Record created2009-07-10
Record modified2020-03-13
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