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| DOI | Resolve DOI: https://doi.org/10.1186/s13104-022-05964-x |
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| Author | Search for: Young, LesterORCID identifier: https://orcid.org/0000-0003-3062-7771; Search for: Akhov, Leonid1; Search for: Kulkarni, Manoj1; Search for: You, Frank; Search for: Booker, Helen |
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| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
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| Funder | Search for: Genome Canada; Search for: Ministry of Agriculture - Saskatchewan |
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| Format | Text, Article |
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| Subject | flax; yellow seed; proanthocyanidin; glutathione S-transferase |
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| Abstract | Objective: The brown seed coat colour of flax (Linum ustiatissimum) results from proanthocyanidin synthesis and accumulation. Glutathione S-transferases (GSTs), such as the TT19 protein in Arabidopsis, have been implicated in the transport of anthocyanidins during the synthesis of the brown proanthocyanidins. This study fine mapped the g allele responsible for yellow seed colour in S95407 and identified it as a putative mutated GST.
Results: We developed a Recombinant Inbred Line population with 320 lines descended from a cross between CDC Bethune (brown seed coat) and S95407 (yellow seed) and used molecular markers to fine map the G gene on Chromosome 6 (Chr 6). We used Next Generation Sequencing (NGS) to identify a putative GST was identified in this region and Sanger sequenced the gene from CDC Bethune, S95407 and other yellow seeded genotypes. The putative GST from S95407 had 13 SNPs encoding, including four non-synonymous amino acid changes, compared to the CDC Bethune reference sequence and the other genotypes. The GST encoded by Lus10019895 is a lambda-GST in contrast to the Arabidopsis TT19 which is a phi-GST. |
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| Publication date | 2022-02-20 |
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| Publisher | BMC |
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| Licence | |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 05254a67-16b6-4d47-9c2f-4d7399cae0b2 |
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| Record created | 2023-12-04 |
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| Record modified | 2023-12-04 |
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