| Download | - View accepted manuscript: Characterization of L-Digitoxosyl-phenanthroviridin from Streptomyces venezuelae ISP5230 (PDF, 979 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1021/acs.jnatprod.5b00277 |
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| Author | Search for: Robertson, Andrew W.; Search for: Martinez-Farina, Camilo F.; Search for: Syvitski, Raymond T.1; Search for: Jakeman, David L. |
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| Affiliation | - National Research Council Canada. Aquatic and Crop Resource Development
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| Format | Text, Article |
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| Subject | aglycone; daunorubicin; digitoxosyl phenanthroviridin; gyrase inhibitor; lysine; unclassified drug; absorption spectroscopy; amino acid metabolism; cancer cell culture; cytotoxicity; fermentation; heteronuclear multiple bond correlation; heteronuclear single quantum coherence; high performance liquid chromatography; human cell; liquid chromatography; mass spectrometry; nuclear Overhauser effect; protein structure; proton nuclear magnetic resonance; Streptomyces venezuelae; tandem mass spectrometry |
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| Abstract | The jadomycin-derived compound l-digitoxosyl-phenanthroviridin was isolated from fermentations of Streptomyces venezuelae ISP5230 grown in nutrient-deficient media with l-lysine as the sole nitrogen source. Structural elucidation was accomplished using a combination of high-resolution MS, LC-MS/MS, and 1D- and 2D-NMR. The compound was evaluated against the National Cancer Institute (NCI) 60 human tumor cell line screen in both the one-dose and five-dose screens, and cytotoxicity was compared to a small library of jadomycin analogues to probe the structure-activity relationship. |
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| Publication date | 2015-07-23 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21277038 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 060c5f22-ee41-4fc5-8909-9e451dc156ac |
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| Record created | 2015-11-10 |
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| Record modified | 2020-06-04 |
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