| DOI | Resolve DOI: https://doi.org/10.1016/j.vaccine.2015.05.097 |
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| Author | Search for: Petiot, Emma; Search for: Cuperlovic-Culf, Miroslava1; Search for: Shen, Chun Fang2; Search for: Kamen, Amine2 |
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| Affiliation | - National Research Council of Canada. Information and Communication Technologies
- National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Article |
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| Abstract | Mammalian cell cultures are increasingly used for the production of complex biopharmaceuticals including viral vectors and vaccines. HEK293 is the predominant cell line used for the transient expression of recombinant proteins and a well-established system for the production of viral vectors. Understanding metabolic requirements for high productivity in HEK293 cells remains an important area of investigation. Many authors have presented approaches for increased productivity through optimization of cellular metabolism from two distinct perspectives. One is a non-targeted approach, which is directed to improving feeding strategies by addition of exhausted or critical substrates and eventually removal of toxic metabolites. Alternatively, a targeted approach has attempted to identify specific targets for optimization through better understanding of the cellular metabolism under different operating conditions. This review will present both approaches and their successes with regards to improvement of viral production in HEK293 cells outlining the key relations between HEK293 cell metabolism and viral vector productivity. Also, we will summarize the current knowledge on HEK293 metabolism indicating remaining issues to address and problems to resolve to maximize the productivity of viral vectors in HEK293 cells. |
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| Publication date | 2015-11-04 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21275745 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 07914c04-0650-4d1c-b1eb-f0e13aab9cde |
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| Record created | 2015-07-14 |
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| Record modified | 2020-04-22 |
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