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Boronate techniques for clean-up and concentration of the vic-diol-containing tetrodotoxins from shellfish

From National Research Council Canada

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AuthorSearch for: 1ORCID identifier: https://orcid.org/0000-0002-5680-2112; Search for: 1; Search for: 1ORCID identifier: https://orcid.org/0000-0002-7746-8432; Search for: ORCID identifier: https://orcid.org/0000-0002-1219-7731; Search for: 1ORCID identifier: https://orcid.org/0000-0003-1644-2121; Search for: ; Search for: ; Search for: ORCID identifier: https://orcid.org/0000-0001-5225-2494; Search for: ; Search for: 1ORCID identifier: https://orcid.org/0000-0001-9840-6874
Affiliation
  1. National Research Council of Canada. Metrology Research Centre
FormatText, Article
ConferenceHarmful Algae 2018 - From Ecosystems to Socio-Ecosystems (ICHA 2018), October 21-26, 2018, Nantes, France
Subjectazaspiracid; tetrodotoxin; boronate; marine biotoxin; LC-MS; matrix effect; sample preparation
Abstract
Boronates bind reversibly to vic-diols, a common structural feature of algal toxins. This boronate–diol interaction can be exploited for selective toxin clean-up and concentration. Boric acid gel (BAG) solid phase extraction (SPE) was recently shown to eliminate interferences and matrix effects in LC-MS analyses of azaspiracids (AZAs) in mussel extracts. Here, we report a modified approach for cleanup of tetrodotoxin (TTX) and many of its congeners, which also contain vic-diols. The reaction between TTX and boronic acids was first investigated in solution to optimize conditions for TTX binding. Then, TTX-contaminated mussel extracts were applied to BAG SPE columns. TTXs were selectively bound and released from the BAG to yield very clean extracts containing TTX analogues and very little else. Potential interferences in LC-MS analyses, such as arginine and other amino acids, were completely eliminated, making this a promising approach for analytical sample preparation.
Publication date
PublisherInternational Society for the Study of Harmful Algae (ISSHA)
Institut Francais de Recherche pour l'Exploitation de la Mer (Ifremer)
In
  • Proceedings of the 18th International Conference on Harmful Algae (ICHA 2018) (2020): 125–128.
LanguageEnglish
Peer reviewedYes
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Record created2024-01-15
Record modified2024-01-15
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