| Subject | acetylation; antigens; carbohydrate conformation; carbohydrate sequence; chromatography, gel; degradation; growth & development; hydrolysis; immunology; isolation & purification; lipopolysaccharides; lipopolysaccharides, smooth; LPS; magnetic resonance spectroscopy; methylation; molecular sequence data; molecular weight; nuclear magnetic resonance, 1D; nuclear magnetic resonance, 2D; O antigens; O-chain; oligosaccharides; polysaccharides; polysaccharides, bacterial; repeating unit; Escherichia coli |
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| Abstract | The structure of the O-polysaccharide of the smooth lipopolysaccharide (LPS) produced by Escherichia coli O64:K99 was investigated by SDS-PAGE, composition, periodate oxidation, methylation, partial hydrolysis, and 1D and 2D nuclear magnetic resonance analyses, made on the native o-chain and its reduction and periodate degradation products. The E. coli O64 antigenic O-chain was found to be a high molecular weight glycan composed of D-galactose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-mannose (2:1:1:1) and was a polymer of branched pentasaccharide repeating units having the structure: [formula: see text] |
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