| DOI | Resolve DOI: https://doi.org/10.1016/j.aca.2017.07.065 |
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| Author | Search for: D'ulivo, Lucia1; Search for: Yang, Lu1; Search for: Ding, Jianfu2; Search for: Pagliano, Enea1; Search for: Leek, Donald M.1; Search for: Thibeault, Marie-Pier1; Search for: Mester, Zoltán1ORCID identifier: https://orcid.org/0000-0002-2377-2615 |
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| Affiliation | - National Research Council of Canada. Measurement Science and Standards
- National Research Council of Canada. Security and Disruptive Technologies
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| Format | Text, Article |
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| Subject | cyanocobalamin; isotope dilution; liquid chromatography mass spectrometry; multivitamins |
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| Abstract | Cyanocobalamin (CNCbl) is an active form of vitamin B12, commonly employed for the preparation of multivitamin supplements and fortified food. In this study, we present a novel analytical method for its determination based on stable isotope dilution liquid chromatography electrospray tandem mass spectrometry (ID LC-MS/MS). Isotopically enriched 13C15NCbl was synthesized in-house and used as internal standard. The method was validated using NIST SRM 3280 multivitamin reference material and by comparison with an independent methodology based on LC-ICPMS. The proposed method provided a detection limit of 57 pg/g and could be applied for the determination of trace level of CNCbl in multivitamin supplements with a relative standard uncertainty better than 3%. The novel ID LC-MS/MS is a primary ratio method that could become a reference for CNCbl determination in multivitamins and food supplements. The method was applied for the characterization of two NRC multivitamin tablet Certified Reference Material (CRM) candidates, VITA-1 and VITB-1 whose CNCbl levels were quantified as 2.64 ± 0.09 and 1.75 ± 0.12 μg/g, respectively. |
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| Publication date | 2017-08-07 |
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| Publisher | Elsevier |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23002343 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 0e50af11-8f2e-4928-89a0-41808abde647 |
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| Record created | 2017-10-20 |
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| Record modified | 2020-03-16 |
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