| Download | - View accepted manuscript: Kinetics of CO conversion into H2 by Carboxydothermus hydrogenoformans (PDF, 654 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1007/s00253-011-3509-7 |
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| Author | Search for: Zhao, Ya1; Search for: Cimpoia, Ruxandra1; Search for: Liu, Zhijun; Search for: Guiot, Serge R.1 |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Abstract | The objective of this study was to improve the biological water-gas shift reaction for producing hydrogen (H2) by conversion of carbon monoxide (CO) using an anaerobic thermophilic pure strain, Carboxydothermus hydrogenoformans. Specific hydrogen production rates and yields were investigated at initial biomass densities varying from 5 to 20 mg volatile suspended solid (VSS) L-1. Results showed that the gas-liquid mass transfer limits the CO conversion rate at high biomass concentrations. At 100-rpm agitation and at CO partial pressure of 1 atm, the optimal substrate/biomass ratio must exceed 5 mol CO g-1 biomass VSS in order to avoid gas-liquid substrate transfer limitation. An average H2 yield of 94±3% and a specific hydrogen production rate of ca. 3 mol g-1 VSS day-1 were obtained at initial biomass densities between 5 and 8 mg VSS-1. In addition, CO bioconversion kinetics was assessed at CO partial pressure from 0.16 to 2 atm, corresponding to a dissolved CO concentration at 70°C from 0.09 to 1.1 mM. Specific bioactivity was maximal at 3.5 mol CO g-1 VSS day-1 for a dissolved CO concentration of 0.55 mM in the culture. This optimal concentration is higher than with most other hydrogenogenic carboxydotrophic species. © 2011 Her Majesty the Queen in Right of Canada. |
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| Publication date | 2011-08-07 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 53371 |
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| NPARC number | 18702532 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 0e6e5f70-b493-4c56-b8d7-8a0147504325 |
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| Record created | 2012-03-06 |
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| Record modified | 2020-04-21 |
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