| DOI | Resolve DOI: https://doi.org/10.1139/o69-164 |
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| Author | Search for: Blakely, J. A.1; Search for: MacKenzie, S. L.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Abstract | A β-hexosidase has been isolated from Sporobolomyces singularis by conventional techniques involving ammonium sulfate precipitation and chromatography on columns of Sephadex G-200 and DEAE-Sephadex A-50. Electrophoresis on polyacrylamide gel was used as the final preparative step. The sedimentation coefficient (s°₂₀,w) of the enzyme was 7.6 and its molecular weight was in the range 140 000–145 000. Although the β-hexosidase performed the functions of a β-D-galactoside galactohydrolase (β-galactosidase), it also catalyzed the hydrolytic function normally performed by a β-D-glucoside glucohydrolase; both these functions appear to reside in the same molecule. |
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| Publication date | 1969-11-01 |
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| Publisher | Canadian Science Publishing |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | NRCC-10910 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 0edb7067-c326-4976-9cee-41a7294b8080 |
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| Record created | 2023-09-06 |
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| Record modified | 2023-09-06 |
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