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Mucosal and systemic immune responses by intranasal immunization using archaeal lipid-adjuvanted vaccines

DOIResolve DOI: https://doi.org/10.1016/j.vaccine.2007.09.042
AuthorSearch for: 1; Search for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council Canada. NRC Institute for Biological Sciences
FormatText, Article
SubjectArchaeal lipid; Mucosal vaccine; Adjuvant
Abstract
The utility of archaeal polar lipids as an adjuvant/delivery system for elicitation of antigen-specific mucosal immune responses in intranasally administered vaccines was investigated. Although unilamellar archaeosomes (liposomes made from archaeal polar lipids) with encapsulated ovalbumin (OVA/archaeosomes) induced anti-OVA IgG antibody responses in sera, they failed to induce anti-OVA IgA antibody responses at mucosal sites. However, the addition of CaCl(2) to convert OVA/archaeosomes into an archaeal lipid mucosal vaccine adjuvant and delivery (AMVAD) vaccine (OVA/AMVAD) consisting of larger, particulate, aggregated structures resulted in an efficacious intranasal (i.n.) vaccine. Intranasal immunization of mice with OVA/AMVAD vaccines prepared from various archaeal polar lipid compositions elicited anti-OVA IgA antibody responses in sera, feces, bile, vaginal and nasal wash samples. The i.n. immunization also induced anti-OVA IgG, IgG1 and IgG2a antibody responses in sera, as well as cytotoxic T lymphocyte responses. The mucosal and systemic immune responses induced by OVA/AMVAD immunization were generally sustained over several months, and were subject to memory boost responses. Thus, polar archaeal lipids appear to be promising for developing a non-replicating mucosal adjuvant and vaccine delivery system
Date published
In
  • Vaccine 25, no. 51 (12 December 2007): 8622–8636.
LanguageEnglish
NRC numberPATEL2007
NPARC number9376207
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Record identifier0f53f21f-b9f2-4be4-ac26-3026ad568264
Record created2009-07-10
Record modified2020-05-10

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