| Download | - View accepted manuscript: Myxoma virus infection of primary human fibroblasts varies with cellular age and is regulated by host interferon responses. (PDF, 1.1 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1016/j.virol.2004.11.030 |
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| Author | Search for: Johnston, J. B.; Search for: Nazarian, Steven H.; Search for: Natale, Renato; Search for: McFadden, Grant |
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| Format | Text, Article |
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| Subject | poxvirus; myxoma virus; tropism; host range; interferon; antiviral responses |
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| Abstract | Recent studies have indicated a critical role for interferon (IFN)-mediated antiviral responses in the host range of myxoma virus (MV), a pathogenic poxvirus of rabbits. To investigate the contribution of IFN to MV tropism in nonleporine cells, primary human dermal fibroblasts (HDFs) were tested for permissiveness to MV infection. Low-passage HDFs that underwent fewer than 25 population doublings (PD) were fully permissive for MV infection, supporting productive virus replication and cell-to-cell spread. In contrast, early and late viral gene expression was detectable in high-passage HDF (>75 PD), but MV failed to generate infectious progeny and could not form foci in these cells. Vesicular stomatitis virus (VSV) plaque reduction assays confirmed that constitutive IFN production progressively increased as HDFs were passaged, concurrent with an increase in the expression of transcripts for type I IFN and IFN-responsive genes involved in antiviral responses. These findings correlated with the enhanced sensitivity of higher-passage HDF to inducers of type I IFN responses, such as dsRNA. Furthermore, pretreatment of low-passage HDF with type I IFN abrogated MV spread and replication while treatment of mature HDF with neutralizing antibodies to IFN-β, but not IFN-α, restored the capacity to form foci. These findings emphasize the importance of post-entry events in determining the permissiveness of human cells to MV infection and support a critical role for innate type I IFN responses as key determinants of poxvirus host range and species restriction. |
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| Publication date | 2004-12-22 |
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| Publisher | Elsevier |
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| Place | Amsterdam |
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| Terms of use | - You may reproduce (print, make photocopies, or download) the accepted manuscript without permission for non-commercial purposes (research, education, and private study), on the condition that you provide proper attribution of the source in all copies.
- Vous pouvez reproduire (imprimer, faire des photocopies ou télécharger) le manuscrit accepté sans autorisation à des fins non commerciales (recherche, éducation et études privées), à condition de fournir la source appropriée dans toutes les copies.
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| Access condition | |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC publication | This is a non-NRC publication"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC. |
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| NPARC number | 8934194 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 0f6a2d35-cb1a-4702-9879-add266d7c461 |
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| Record created | 2009-04-23 |
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| Record modified | 2020-05-08 |
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