| Download | - View accepted manuscript: Biosynthesis of bikaverin in Fusarium oxysporum: use of ¹³C nuclear magnetic resonance with homonuclear ¹³C decoupling to locate adjacent ¹³C labels. (PDF, 1.2 MiB)
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| DOI | Resolve DOI: https://doi.org/10.7164/antibiotics.29.1050 |
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| Author | Search for: MCINNES, A. G.1; Search for: J. A. WALTER1; Search for: D. G. SMITH1; Search for: WRIGHT, J. L. C.1; Search for: VINING, L. C. |
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| Affiliation | - National Research Council of Canada. Atlantic Regional Laboratory
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| Format | Text, Article |
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| Abstract | Bikaverin obtained by supplementing cultures of Fusarium oxysporum with singly and doubly ¹³C labeled acetate was enriched by approximately 0.5 atom percent with the 13C isotope. At this low enrichment ¹³C NMR spectra of samples labeled from (1-¹³C)- and (2-¹³C) acetate did not show, unequivocally, the pattern of isotopic incorporation. Small sample size, poor solubility and difficulties in the assignment of resonances also restricted the amount of information that could be obtained from the ¹³C NMR spectrum of the sample labeled from (1, 2-¹³C) acetate. The difficulty was overcome by using ¹³C homonuclear single-frequency decoupling in conjunction with ¹H heteronuclear decoupling to locate bonded ¹³C-¹³C pairs. The carbon skeleton of bikaverin was shown to be biosynthesized entirely by condensation of acetate units and the pattern of assembly was established. |
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| Publication date | 1976-10 |
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| Publisher | Japan Antibiotics Research Association |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC-15545 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 0f6feb8a-e33b-4e08-b54f-5de75355c055 |
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| Record created | 2023-06-28 |
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| Record modified | 2024-05-21 |
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