| DOI | Resolve DOI: https://doi.org/10.1364/OE.17.002984 |
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| Author | Search for: Pegoraro, Adrian F.1; Search for: Ridsdale, Andrew1; Search for: Moffatt, Douglas J.1; Search for: Jia, Yiwei; Search for: Pezacki, John Paul1; Search for: Stolow, Albert1 |
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| Affiliation | - National Research Council of Canada. NRC Steacie Institute for Molecular Sciences
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| Format | Text, Article |
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| Subject | CARS; microscopy; live cells; femtosecond; photonic crystal fiber; Spectral chirp; laser pulses; multimodal |
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| Abstract | We demonstrate high performance coherent anti-Stokes Raman scattering (CARS) microscopy of live cells and tissues with user-variable spectral resolution and broad Raman tunability (2500 - 4100 cm-1), using a femtosecond Ti:Sapphire pump and photonic crystal fiber output for the broadband synchronized Stokes pulse. Spectral chirp of the fs laser pulses was a user-variable parameter for optimization in a spectral focusing implementation of multimodal CARS microscopy. High signal-to-noise, high contrast multimodal imaging of live cells and tissues was achieved with pixel dwell times of 2-8 µs and low laser powers (< 30 mW total). |
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| Publication date | 2009-02-16 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 16488518 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 10888da2-65c4-452b-bf25-c6627525f950 |
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| Record created | 2011-02-15 |
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| Record modified | 2020-04-16 |
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