| Download | - View author's version: Chromosome inner structure investigation by electron tomography and electron diffraction in a transmission electron microscope (PDF, 4.4 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1007/s10577-021-09661-6 |
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| Author | Search for: Phengchat, Rinyaporn1ORCID identifier: https://orcid.org/0000-0003-1128-8209; Search for: Malac, Marek1; Search for: Hayashida, Misa1 |
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| Affiliation | - National Research Council Canada. Nanotechnology
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| Format | Text, Article |
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| Subject | chromosome structure; electron tomography; electron diffraction; chromosome scaffold; transmission electron microscope |
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| Abstract | Our understanding of the inner structure of metaphase chromosomes remains inconclusive despite intensive studies using multiple imaging techniques. Transmission electron microscopy has been extensively used to visualize chromosome ultrastructure. This review summarizes recent results obtained using two transmission electron microscopy-based techniques: electron tomography and electron diffraction. Electron tomography allows advanced three-dimensional imaging of chromosomes, while electron diffraction detects the presence of periodic structures within chromosomes. The combination of these two techniques provides results contributing to the understanding of local structural organization of chromatin fibers within chromosomes. |
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| Publication date | 2021-03-18 |
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| Publisher | Springer |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRC-NANO 149 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 12837488-04e4-4512-b8af-df22d58a0911 |
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| Record created | 2021-05-07 |
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| Record modified | 2021-09-21 |
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