Structural characterization of wall and lipidated polysaccharides from Clostridium perfringens ATCC 13124

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.carres.2017.06.003
AuthorSearch for: 1; Search for: 1; Search for: 1
Name affiliation
  1. National Research Council of Canada. Human Health Therapeutics
FormatText, Article
Journal titleCarbohydrate Research
ISSN0008-6215
1873-426X
Volume448
Pages88–94
Subjectclostridium perfringens; NMR spectroscopy; polysaccharide; structure
Abstract
Cell surface polysaccharides produced by C. perfringens ATCC 13124 were analyzed using NMR, chemical and immunological methods. Two distinct polymers were identified. The more abundant PS1 had a structure based on a polymer of β-mannosamine with a number of modifications, including varying levels of substitution at O-6 with PEtN, N-acetylation, and different linkages between monosaccharides. The shortest variant of PS1 represented a lipoteichoic acid. It contained only 1-4-linkages between ManNAc residues, minor branching α-Ribf, and glucosyl-glycerol at the reducing end, which was acylated with linear saturated fatty acids C16, C18, and C20 (dominant). Other non-lipidated variants of PS1 contained less PEtN, no α-Ribf, up to 50% 1-3-linkages, and up to 25% ManN with the free amino group. The minor polysaccharide PS2 had a linear regular structure with a -4-α-Rha-3-β-Gal-4-β-GalNAc3PCho- repeating unit, where PCho indicates phosphocholine.
Publication date
PublisherElsevier
LanguageEnglish
Peer reviewedYes
NPARC number23002063
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Record created2017-08-01
Record modified2020-03-16
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