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Dynamics of lipid droplets induced by the hepatitis C virus core protein

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.bbrc.2010.07.101
AuthorSearch for: 1; Search for: 1; Search for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Steacie Institute for Molecular Sciences
FormatText, Article
Subjectdynamics of lipid droplets; hepatitis C virus; protein; CARS microscopy; Particle tracking; Dynamics; Microtubules
Abstract
The hepatitis C virus (HCV) is a global health problem, with limited treatment options and no vaccine available. HCV uses components of the host cell to proliferate, including lipid droplets (LD) onto which HCV core proteins bind and facilitate viral particle assembly. We have measured the dynamics of HCV core protein-mediated changes in LDs and rates of LD movement on microtubules using a combination of coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence (TPF), and differential interference contrast (DIC) microscopies. Results show that the HCV core protein induces rapid increases in LD size. Particle tracking experiments show that HCV core protein slowly affects LD localization by controlling the directionality of LD movement on microtubules. These dynamic processes ultimately aid HCV in propagating and the molecules and interactions involved represent novel targets for potential therapeutic intervention.
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In
  • Biochemical and Biophysical Research Communications 399 (1 August 2010): 518–524.
LanguageEnglish
Peer reviewedYes
NRC publication
This is a non-NRC publication

"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.

NPARC number16925469
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Record identifier198291d4-c59e-4f35-b6d7-cd387ba49765
Record created2011-02-25
Record modified2020-04-17
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