| DOI | Resolve DOI: https://doi.org/10.1128/AEM.02170-20 |
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| Author | Search for: Correa-García, Sara; Search for: Rheault, Karelle; Search for: Tremblay, Julien1; Search for: Séguin, Armand; Search for: Yergeau, Etienne |
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| Editor | Search for: Cann, Isaac |
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| Affiliation | - National Research Council of Canada. Energy, Mining and Environment
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| Funder | Search for: Natural Sciences and Engineering Research Council of Canada |
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| Format | Text, Article |
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| Subject | phenanthrene; phytoremediation; polycyclic aromatic hydrocarbons; poplar; rhizosphere-inhabiting microbes; soil contamination; soil diversity |
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| Abstract | Rhizodegradation is a promising cleanup technology where microorganisms degrade soil contaminants in the rhizosphere. A symbiotic relationship is expected to occur between plant roots and soil microorganisms in contaminated soils that enhances natural microbial degradation. However, little is known about how different initial microbiotas influence the rhizodegradation outcome. Recent studies have hinted that soil initial diversity has a determining effect on the outcome of contaminant degradation. To test this, we either planted (P) or not (NP) balsam poplars (Populus balsamifera) in two soils of contrasting diversity (agricultural and forest) that were contaminated or not with 50 mg kg⁻¹ of phenanthrene (PHE). The DNA from the rhizosphere of the P and the bulk soil of the NP pots was extracted and the bacterial genes encoding the 16S rRNA, the PAH ring-hydroxylating dioxygenase alpha subunits (PAH-RHDα) of Gram-positive and Gram-negative bacteria, and the fungal ITS region were sequenced to characterize the microbial communities. The abundances of the PAH-RHDα genes were quantified by real-time quantitative PCR. Plant presence had a significant effect on PHE degradation only in the forest soil, whereas both NP and P agricultural soils degraded the same amount of PHE. Fungal communities were mainly affected by plant presence, whereas bacterial communities were principally affected by the soil type, and upon contamination the dominant PAH-degrading community was similarly constrained by soil type. Our results highlight the crucial importance of soil microbial and physicochemical characteristics in the outcome of rhizoremediation. |
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| Publication date | 2021-01-04 |
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| Publisher | American Society for Microbiology |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 1c71902a-34bd-47a2-aaff-2ef7867c6d3a |
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| Record created | 2023-04-27 |
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| Record modified | 2023-04-27 |
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