| Abstract | The proton magnetic resonance spectra of the mannose-containing polysaccharides from some new species of Hansenula were obtained and used as an aid in establishing their phylogenetic relationships to other members of the genus. The spectrum of the mannan from Hansenula dimennae is identical with that of the mannan from Hansenula californica. These two haploid species are taxonomically closely related and are adjacent on the same line of the phylogenetic diagram according to Wickerham. Hansenula saturnus var. subsufficiens mannan has a spectrum like those of the mannans of some strains of Hansenula mrakii and Hansenula beijerinckii. The new species Hansenula henricii, Hansenula nonfermentans, and Hansenula glucozyma all form mannans with spectra having characteristic signals at about τ4.24 and τ4.38, as do the spectra of the mannans formed by the other species on the same phylogenetic line, Hansenula polymorpha, Hansenula minuta, and Hansenula wickerhamii. These species have, relatively, DNA's with the highest guanine and cytosine percentages in the genus. Hansenula platypodis, a species which, unlike other Hansenula species, forms ascophores and anastomoses, has DNA with a lower content of guanine plus cytosine. The spectrum of H. platypodis mannan is like that of H. minuta mannan, with the exception of two small signals at τ4.44 and τ4.48 which do not occur in the spectrum of H. minuta mannan. |
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