| DOI | Resolve DOI: https://doi.org/10.1534/genetics.110.124925 |
|---|
| Author | Search for: Navabi, Z.K.; Search for: Stead, K.E.; Search for: Pires, J.C.; Search for: Xiong, Z.; Search for: Sharpe, A.G.1; Search for: Parkin, I.A.P.; Search for: Rahman, M.H.; Search for: Good, A.G. |
|---|
| Affiliation | - National Research Council of Canada. NRC Plant Biotechnology Institute
|
|---|
| Format | Text, Article |
|---|
| Subject | agronomic trait; chromosome analysis; chromosome pairing; chromosome rearrangement; gene mapping; genetic linkage; genetic marker; genetic recombination; genome analysis; genomic in situ hybridization; inheritance; introgression; morphological trait; plant chromosome; plant genetics; plant genome; rapeseed; root vegetable; tetraploidy; Chimera; genetic linkage; genome, plant; hybridization, genetic; in situ hybridization, fluorescence; minisatellite repeats; polyploidy; Brassica carinata; Brassica; Brassica carinata; Brassica napus |
|---|
| Abstract | Brassica carinata, an allotetraploid with B and C genomes, has a number of traits that would be valuable to introgress into B. napus. Interspecific hybrids were created between B. carinata (BBCC) and B. napus (AACC), using an advanced backcross approach to identify and introgress traits of agronomic interest from the B. carinata genome and to study the genetic changes that occur during the introgression process. We mapped the B and C genomes of B. carinata with SSR markers and observed their introgression into B. napus through a number of backcross generations, focusing on a BC3 and BC3S 1 sibling family. There was close colinearity between the C genomes of B. carinata and B. napus and we provide evidence that B. carinata C chromosomes pair and recombine normally with those of B. napus, suggesting that similar to other Brassica allotetraploids no major chromosomal rearrangements have taken place since the formation of B. carinata. There was no evidence of introgression of the B chromosomes into the A or C chromosomes of B. napus; instead they were inherited as whole linkage groups with the occasional loss of terminal segments and several of the B-genome chromosomes were retained across generations. Several BC3S1 families were analyzed using SSR markers, genomic in situ hybridization (GISH) assays, and chromosome counts to study the inheritance of the B-genome chromosome(s) and their association with morphological traits. Our work provides an analysis of the behavior of chromosomes in an interspecific cross and reinforces the challenges of introgressing novel traits into crop plants. Copyright © 2011 by the Genetics Society of America. |
|---|
| Publication date | 2011 |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NPARC number | 21271381 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 1f1fae04-b542-413f-b587-41283463c3ad |
|---|
| Record created | 2014-03-24 |
|---|
| Record modified | 2023-01-23 |
|---|
