The chromatin regulator Brpf1 regulates embryo development and cell proliferation

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1074/jbc.M115.643189
Author	Search for: You, Linya; Search for: Yan, Kezhi; Search for: Zou, Jinfeng¹; Search for: Zhao, Hong; Search for: Bertos, Nicholas R.; Search for: Park, Morag; Search for: Wang, Edwin; Search for: Yang, Xiang-Jiao
Affiliation	National Research Council of Canada. Human Health Therapeutics
Format	Text, Article
Subject	ablation; cell culture; cell proliferation; chromosomes; gene expression; mammals; mobile security; proteins; transcription; complex formations; embryonic fibroblasts; embryonic lethality; histone modification; pathological process; ribosomal proteins; substrate specificity; systematic analysis; bromodomain and PHD finger containing protein 1; genomic DNA; membrane protein; nuclear protein; protein p16; protein p27; protein Scp3; regulator protein; animal cell; animal tissue; cell proliferation; embryo cell; embryo development; embryonic fibroblast; embryopathy; fibroblast; gene; gene expression regulation; growth inhibition; hematopoietic stem cell; immunohistochemistry; mouse; mouse mutant; neural tube defect; placenta disorder; protein analysis; protein depletion; protein expression; protein function; ribosomal protein L10 like gene; survival rate; transcription regulation; yolk sac
Abstract	With hundreds of chromatin regulators identified in mammals, an emerging issue is how they modulate biological and pathological processes. BRPF1 (bromodomain-and PHD finger-containing protein 1) is a unique chromatin regulator possessing two PHD fingers, one bromodomain and a PWWP domain for recognizing multiple histone modifications. In addition, it binds to the acetyltransferases MOZ, MORF, and HBO1 (also known as KAT6A, KAT6B, and KAT7, respectively) to promote complex formation, restrict substrate specificity, and enhance enzymatic activity.Wehave recently showed that ablation of the mouse Brpf1 gene causes embryonic lethality at E9.5. Here we present systematic analyses of the mutant animals and demonstrate that the ablation leads to vascular defects in the placenta, yolk sac, and embryo proper, as well as abnormal neural tube closure. At the cellular level, Brpf1 loss inhibits proliferation of embryonic fibroblasts and hematopoietic progenitors. Molecularly, the loss reduces transcription of a ribosomal protein L10 (Rpl10)-like gene and the cell cycle inhibitor p27, and increases expression of the cell-cycle inhibitor p16 and a novel protein homologous to Scp3, a synaptonemal complex protein critical for chromosome association and embryo survival. These results uncover a crucial role of Brpf1 in controlling mouse embryo development and regulating cellular and gene expression programs.
Publication date	2015-03-15
Publisher	American Society for Biochemistry and Molecular Biology
In	The Journal of Biological Chemistry 290, no. 18: 11349–11364.
Language	English
Peer reviewed	Yes
NPARC number	21275666
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Record identifier	1f8f04fa-88fc-417e-94f8-3eefc442af12
Record created	2015-07-14
Record modified	2020-04-22

Date modified:: 2024-12-26