| Download | - View final version: Second harmonic generation microscopy: a powerful tool for bio-imaging (PDF, 50.4 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1007/s12551-022-01041-6 |
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| Author | Search for: Aghigh, ArashORCID identifier: https://orcid.org/0000-0001-6205-4707; Search for: Bancelin, Stéphane; Search for: Rivard, Maxime1; Search for: Pinsard, Maxime; Search for: Ibrahim, Heide; Search for: Légaré, François |
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| Affiliation | - National Research Council of Canada. Digital Technologies
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| Funder | Search for: Natural Sciences and Engineering Research Council of Canada; Search for: New Frontiers Research Fund; Search for: Canada Foundation of Innovation; Search for: Natural Sciences and Engineering Research Council of Canada. CREATE program |
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| Format | Text, Article |
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| Subject | non-linear microscopy; SHG; neuroimaging; interferometry; polarimetry |
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| Abstract | Second harmonic generation (SHG) microscopy is an important optical imaging technique in a variety of applications. This article describes the history and physical principles of SHG microscopy and its more advanced variants, as well as their strengths and weaknesses in biomedical applications. It also provides an overview of SHG and advanced SHG imaging in neuroscience and microtubule imaging and how these methods can aid in understanding microtubule formation, structuration, and involvement in neuronal function. Finally, we offer a perspective on the future of these methods and how technological advancements can help make SHG microscopy a more widely adopted imaging technique. |
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| Publication date | 2023-01-19 |
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| Publisher | Springer |
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| Licence | |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 20317ea2-40bf-49ff-93b4-609cb6dcd355 |
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| Record created | 2023-01-31 |
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| Record modified | 2024-04-26 |
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