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Free-solution electrophoresis of DNA

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/S0021-9673(97)00656-0
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ORCID identifier: https://orcid.org/0000-0002-0665-7041; Search for: ; Search for:
FormatText, Article
SubjectDNA; free solution capillary electrophoresis
Abstract
We report the first experimental evidence that double-stranded DNA fragments can be electrophoretically separated in free-solution (i.e., in the absence of a sieving matrix) when either a single or two streptavidin molecules are attached to the end(s) using biotinylated nucleotides. As previously predicted, higher resolution is obtained at higher electric fields or when two streptavidin molecules are attached to each DNA fragment. The resolution is also affected by the diameter and coating of the capillaries.
Publication date
PublisherElsevier
In
  • Journal of Chromatography A 806, no. 1 (May 1998): 113–121.
LanguageEnglish
Peer reviewedYes
NRC publication
This is a non-NRC publication

"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.

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Record identifier258086ac-d4e0-44e2-9ee9-311afcee02dd
Record created2024-07-09
Record modified2024-07-09
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