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Development of sensitive LCMS methods for the evaluation of excitotoxic amino acids in marine algae

From National Research Council Canada

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Affiliation
  1. National Research Council Canada. Aquatic and Crop Resource Development
  2. National Research Council Canada. Measurement Science and Standards
FormatText, Address
Conference12th International Conference on Harmful Algae,4-8 September 2006, Copenhagen, Denmark
Subjectmass spectrometry; liquid chromatography; amino acids; marine algae; algae; excitotoxic
Abstract
A novel hydrophilic interaction liquid chromatography mass spectrometry (HILIC-MS) method was developed for the separation of excitatory amino acids (EAA) including kainic acid, domoic acid, glutamic acid, aspartic acid, ß-oxalylamino-Lalanine and L-ß-methylaminoalanine in a variety of marine algae. Several HILIC stationary phases were assessed during this study including TSK-gel amide-80 (Tosoh), Atlantis HILIC silica (Waters) and the highly polar, zwitterionic ZIC HILIC (SeQuant). In each case, a variety of parameters were optimised including buffer concentration, organic modifiers, ion-pairing agents, pH, column temperature and flow rate. Single ion monitoring (SIM) of the positive and negative parent ions was performed using an API III, API 165 and API 4000 mass spectrometer. Positive and negative MS/MS spectra were obtained for each EAA examined with the API4000 triple quadrupole MS which facilitated optimization of collision energy and declustering potentials of major fragment ions for single and multiple reaction monitoring. All aspects of quantification will be discussed including linearity assessment, level of detection, level of quantification and effects of sample matrix for each EAA under investigation. These studies were applied to purity assessment of algal derived products such as kainic and domoic acid, and excitotoxin identification in seaweeds, diatoms and cyanobacteria
LanguageEnglish
Peer reviewedNo
NRC numberNRCC 42621
NRC-IMB-1466
NPARC number3538354
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Record identifier270955af-1c38-4094-b1e1-196292ebfe28
Record created2009-03-01
Record modified2020-03-03
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