| Abstract | Glucosinolates were detected as their aglycones in cell suspension cultures of two of seven plant species known to contain glucosinolates. 2-Phenylethyl-glucosinolate and 2-hydroxy-2-phenylethylglucosinolate were present in cell cultures of Reseda luteola and benzylglucosinolate was found in Tropaeolum manus cultures. The glucosinolate-hydrolyzing enzyme, myrosinase (E.C. 3.2.3.1), was detected in all seven cultures. Although o glucosinolates were detected in cultures of Sinapis alba. retention, in part, of biosynthetic capability was demonstrated by the occurrence of a glucosyltransferase associated with gluconsinolate biosynthesis. It is suggested that plant tissue cultures may be of potential value for further study of glucosinolate biosynthesis. |
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