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A rapid, simple method for nuclei isolation from plant protoplasts

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1104/pp.60.2.179
AuthorSearch for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. Prairie Regional Laboratory
FormatText, Article
Abstract
A rapid, simple method for nuclei isolation and purification from soybean (Glycine max L. Merr.) protoplasts is described. The isolated nuclei exhibited active amino acid incorporation and RNA synthesis, but DNA synthesis was not detectable. Analysis by CsCl density gradient centrifugation showed that DNA isolated from nuclei had a single band, while DNA isolated from protoplasts consisted of three bands comprised of nuclear DNA, mitochondrial DNA, and chloroplast DNA.
Publication date
PublisherOxford University Press
In
  • Plant Physiology 60, no. 2 (1 August 1977): 179–181.
LanguageEnglish
Peer reviewedYes
NRC numberNRCC-15964
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Record identifier291ea5f6-e72a-45db-b2e0-4b32f071e408
Record created2023-07-06
Record modified2023-07-11
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