| Download | - View final version: A method for structure–activity analysis of quorum-sensing signaling peptides from naturally transformable streptococci (PDF, 822 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1007/s12575-009-9009-9 |
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| Author | Search for: Tian, Xiaolin; Search for: Syvitski, Raymond T.1; Search for: Liu, Tianlei; Search for: Livingstone, Nadine; Search for: Jakeman, David L.1; Search for: Li, Yung-Hua1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Marine Biosciences
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| Format | Text, Article |
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| Subject | quorum sensing; signaling peptides; structure–activity analysis; circular dichroism and nuclear magnetic resonance spectroscopy; streptococcus mutans |
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| Abstract | Many species of streptococci secrete and use a competence-stimulating peptide (CSP) to initiate quorum sensing for induction of genetic competence, bacteriocin production, and other activities. These signaling molecules are small, unmodified peptides that induce powerful strain-specific activity at nano-molar concentrations. This feature has provided an excellent opportunity to explore their structure–function relationships. However, CSP variants have also been identified in many species, and each specifically activates its cognate receptor. How such minor changes dramatically affect the specificity of these peptides remains unclear. Structure–activity analysis of these peptides may provide clues for understanding the specificity of signaling peptide–receptor interactions. Here, we use the Streptococcus mutans CSP as an example to describe methods of analyzing its structure–activity relationship. The methods described here may provide a platform for studying quorum-sensing signaling peptides of other naturally transformable streptococci. |
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| Publication date | 2009-06-11 |
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| Publisher | BMC |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23004426 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 2961502d-99c5-4677-afc7-8f60c92f0991 |
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| Record created | 2018-11-01 |
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| Record modified | 2020-05-30 |
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