| Download | - View final version: Efficiency of CRISPR-Cas9 genetic engineering in Escherichia coli BL21 is impaired by lack of Lon protease (PDF, 522 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1016/j.mimet.2022.106648 |
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| Author | Search for: Okshevsky, Mira1; Search for: Xu, Yali1; Search for: Masson, Luke1; Search for: Arbour, Melanie1 |
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| Affiliation | - National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Article |
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| Subject | CRISPR-Cas9; Escherichia coli BL21; Lon protease; genetic engineering |
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| Abstract | The efficiency with which E.coli BL21 can be modified using CRISPR-Cas9 genetic engineering is several orders of magnitude lower than that of E. coli W3110. We show that the lack of Lon protease is responsible, and demonstrate that restoration of the Lon protease or knock-out of sulA improves CRISPR-Cas9 engineering efficiency of BL21 to levels comparable to E. coli W3110. |
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| Publication date | 2022-12-05 |
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| Publisher | Elsevier |
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| Licence | |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | S0167701222002433 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 29a2e67d-4d09-4f1d-bb8e-5b2a3cdb2979 |
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| Record created | 2023-09-14 |
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| Record modified | 2023-09-14 |
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