| DOI | Resolve DOI: https://doi.org/10.1016/j.watres.2013.11.047 |
|---|
| Author | Search for: Biswal, B.K.; Search for: Mazza, A.1; Search for: Masson, L.1; Search for: Gehr, R.; Search for: Frigon, D. |
|---|
| Affiliation | - National Research Council of Canada
|
|---|
| Format | Text, Article |
|---|
| Subject | antimicrobial resistances; DNA micro-array; insertion sequences; physicochemical; bioassay; biochips; effluent treatment; effluents; microorganisms; wastewater treatment; aminoglycoside; antibiotic agent; beta lactam antibiotic; integrase; quinolone; tetracycline derivative; transposase; activated sludge; disinfection; fecal coliform; genes; genome; genotype; public health; sewage treatment; virulence; activated sludge process; antibiotic resistance; bacterial gene; bacterial genome; bacterial virulence; bacterium isolation; DNA microarray; DNA sequence; gene insertion; genotyping technique; influent; physical chemistry; waste water management; waste water treatment plant; water pollutant; Escherichia coli |
|---|
| Abstract | An increase in the frequency of antimicrobial resistance genes (ARGs) in bacteria including Escherichia coli could be a threat to public health. This study investigated the impact of activated sludge and physicochemical wastewater treatment processes on the prevalence of ARGs in E. coli isolates. In total, 719 E. coli were isolated from the influent and effluent (prior to disinfection) of two activated sludge and two physicochemical municipal treatment plants, and genotyped using DNA microarrays. Changes in the abundance of ARGs in the E. coli population were different for the two treatment processes. Activated sludge treatment did not change the prevalence of ARG-possessing E. coli but increased the abundance of ARGs in the E. coli genome while physicochemical treatment reduced both the prevalence of ARG-carrying E. coli as well as the frequency of ARGs in the E. coli genome. Most E. coli isolates from the four treatment plants possessed ARGs of multiple antimicrobial classes, mainly aminoglycoside, β-lactams, quinolone and tetracyclines. In addition these isolates harboured DNA insertion sequence elements including integrase and transposase. A significant positive association was found between the occurrence of ARGs and virulence genotypes. © 2013 Elsevier Ltd. |
|---|
| Publication date | 2014 |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NPARC number | 21270830 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 29cadd30-4e40-443b-977a-99280f37691c |
|---|
| Record created | 2014-02-17 |
|---|
| Record modified | 2022-11-18 |
|---|
