Classical immunoproteomics: serological proteome analysis (SERPA) for antigen identification

DOI	Resolve DOI: https://doi.org/10.1007/978-1-4939-9597-4_3
Author	Search for: Fulton, Kelly M.¹; Search for: Ananchenko, Anna¹; Search for: Wolfraim, Lawrence; Search for: Martin, Shannon; Search for: Twine, Susan M.¹
Affiliation	National Research Council of Canada. Human Health Therapeutics
Format	Text, Book Chapter
Subject	SERPA; serological proteome analysis; western blotting; immunoproteomics; comparative immunoproteomics; Francisella; tularemia; live vaccine strain; immunoreactivity; antigens; biomarkers
Abstract	The study of the humoral immune response to infectious and chronic diseases is important for understanding the disease progression, identification of protective antigens, vaccine development, and discovery of biomarkers for early diagnosis. Proteomic approaches, including serological proteome analysis (SERPA), have been used to identify the repertoire of immunoreactive proteins in various diseases. In this chapter, we provide an outline of the SERPA approach, using the analysis of sera from mice vaccinated with a live attenuated tularemia vaccine as an example.
Publication date	2019-07-31
Publisher	Springer
In	Immunoproteomics: 59–78.
Series	Methods in Molecular Biology 2024.
Language	English
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Record identifier	2d35d23f-89c1-43b2-a06e-ee9bdcf2d58b
Record created	2021-02-16
Record modified	2021-02-16