A mussel tissue certified reference material for multiple phycotoxins. Part 4: certification

From National Research Council Canada

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DOIResolve DOI: https://doi.org/10.1007/s00216-016-0004-0
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  1. National Research Council Canada. Measurement Science and Standards
FormatText
TypeArticle
Journal titleAnalytical and Bioanalytical Chemistry
ISSN1618-2642
1618-2650
SubjectAzaspiracid; Okadaic acid; Dinophysistoxin; Pectenotoxin; Yessotoxin; Spirolide; Domoic acid; Mytilus edulis
Abstract
A freeze-dried mussel tissue (Mytilus edulis) reference material (CRM-FDMT1) was produced containing multiple groups of shellfish toxins. Homogeneity and stability testing showed the material to be fit for purpose. The next phase of work was to assign certified values and uncertainties to 10 analytes from six different toxin groups. Efforts involved optimizing extraction procedures for the various toxin groups and performing measurements using liquid chromatography-based analytical methods. A key aspect of the work was compensating for matrix effects associated with liquid chromatography-mass spectrometry through standard addition, dilution, or matrix-matched calibration. Certified mass fraction values are reported as mg/kg of CRM-FDMT1 powder as bottled for azaspiracid-1, -2, and -3 (4.10 ± 0.40; 1.13± 0.10; 0.96 ± 0.10, respectively), okadaic acid, dinophysistoxin-1 and -2 (1.59 ± 0.18; 0.68 ± 0.07; 3.57± 0.33, respectively), yessotoxin (2.49 ± 0.28), pectenotoxin-2 (0.66 ± 0.06), 13-desmethylspirolide-C (2.70 ± 0.26), and domoic acid (126 ± 10). Combined uncertainties for the certified values include contributions from homogeneity, stability, and characterization experiments. The commutability of CRM-FDMT1 was assessed by examining the extractability and matrix effects for the freeze-dried material in comparison with its equivalent wet tissue homogenate. CRM-FDMT1 is the first shellfish matrix CRM with certified values for yessotoxins, pectenotoxins or spirolides, and is the first CRM certified for multiple toxin groups. CRM-FDMT1 is a valuable tool for quality assurance of phycotoxin monitoring programs and for analytical method development and validation.
Publication date
PublisherSpringer
LanguageEnglish
Peer reviewedYes
NPARC number23000974
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Record identifier2d72a8c8-d862-4bae-ae12-3ba2bf19d83e
Record created2016-11-23
Record modified2020-03-16
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