DOI | Resolve DOI: https://doi.org/10.1166/jnn.2012.6449 |
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Author | Search for: Hao, Yuzhi1; Search for: Yang, Xiaoyan; Search for: Shi, Yongzhong; Search for: Xing, James; Search for: Marowitch, Janet; Search for: Chen, Jianmin; Search for: Chen, Jie1 |
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Affiliation | - National Research Council of Canada. Security and Disruptive Technologies
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Format | Text, Article |
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Subject | Covalently bonded; Endosomes; FITC Delivery; Fluorescein isothiocyanate; Magnetic force; Magnetic Single-Walled Carbon Nanotubes (SWCNTs); Nuclear membranes; Plant cells; Protoplast of Canola and Carrot; TEM images; Cell membranes; Plant cell culture; Single-walled carbon nanotubes (SWCN) |
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Abstract | In this paper, fluoresce in isothiocyanate (FITC) was covalently bonded with magnetic single-walled carbon nanotubes (mSWCNTs) that were purified using our previous method. To demonstrate our design, mSWCNT-FITC was delivered into plant cells (canola and carrot cells) driven by external magnetic forces. From FACS results, the FITC delivery efficiency was about 100% for both two canola and carrot protoplasts, which were further confirmed by the confocal and sectional TEM images. Some mSWCNTs were found trapped both inside the endosomes of canola protoplast and outside endosome near the nuclear membrane of carrot protoplast according to the sectional TEM images. All results showed that mSWCNT is a good delivery carrier for biomolecules. |
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Publication date | 2012-08 |
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In | |
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Language | English |
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Peer reviewed | Yes |
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NPARC number | 21270230 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | 2fc29e6c-1675-4e3c-8ad5-9751c6419f2d |
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Record created | 2014-01-14 |
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Record modified | 2020-04-21 |
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