Mutations conferring resistance to azide in Escherichia coli occur primarily in the secA gene

Author	Search for: Fortin, Y.; Search for: Phoenix, P.; Search for: Drapeau, G. R.
Format	Text, Article
Abstract	Mutant strains of Escherichia coli were screened for the ability to grow on L agar plates containing 3.4 or 4.6 mM sodium azide. Most mutants had mutations located in the leucine region, presumably at the azi locus. Two of these mutants were found to have a mutation in the secA gene, but expression of the resistance phenotype also required the presence of upstream gene X. While a plasmid carrying the X-secA mutant gene pair was able to confer azide resistance to a sensitive host, a similar plasmid harboring the wild-type secA allele rendered a resistant strain sensitive to azide, indicating codominance of the two alleles. That azide inhibits SecA is consistent with the fact that SecA has ATPase activity, an activity that is often prone to inhibition by azide.
Publication date	1990-11
In	Journal of Bacteriology 172, no. 11 (November 1990): 6607–6610.
Language	English
NRC publication	This is a non-NRC publication "Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.
NPARC number	12328442
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Record identifier	3397a7a0-6ab2-44a7-99f1-ed62f45cc590
Record created	2009-09-10
Record modified	2020-03-17