| Abstract | A method has been developed, based on polarized fluorescence intensity measurements, for studying the orientation and motion of fluorescent probes incorporated into lipid bilayers. Its use is illustrated in a study of the effect of cholesterol in egg lecithin multibilayers. When no cholesterol is present the probes are constrained to angles between 0° and 80° to the normal to the plane and show, superimposed, a rapid but restricted motion. The angular distribution decreases to a limiting range of 0° to 67° as cholesterol is increased to 25–30 mole % but the rapid motion is unaffected. |
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