Phylogenetic and molecular characterization of a 23S rRNA gene positions the genus Campylobacter in the epsilon subdivision of the Proteobacteria and shows that the presence of transcribed spacers is common in Campylobacter spp.

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Author	Search for: Trust, T. J.; Search for: Logan, S. M.; Search for: Gustafson, C. E.; Search for: Romaniuk, P. J.; Search for: Kim, N. W.; Search for: Chan, V. L.; Search for: Ragan, M. A.¹ ; Search for: Guerry, P.; Search for: Gutell, R. R.
Name affiliation	National Research Council Canada. NRC Institute for Marine Biosciences
Format	Text
Type	Article
Journal title	Journal of Bacteriology
ISSN	0021-9193 1098-5530
Volume	176
Issue	15
Pages	4597–4609
Abstract	The nucleotide sequence of a 23S rRNA gene of Campylobacter coli VC167 was determined. The primary sequence of the C. coli 23S rRNA was deduced, and a secondary-structure model was constructed. Comparison with Escherichia coli 23S rRNA showed a major difference in the C. coli rRNA at approximately position 1170 (E. coli numbering) in the form of an extra sequence block approximately 147 bp long. PCR analysis of 31 other strains of C. coli and C. jejuni showed that 69% carried a transcribed spacer of either ca. 147 or ca. 37 bp. Comparison of all sequenced Campylobacter transcribed spacers showed that the Campylobacter inserts were related in sequence and percent G+C content. All Campylobacter strains carrying transcribed spacers in their 23S rRNA genes produced fragmented 23S rRNAs. Other strains which produced unfragmented 23S rRNAs did not appear to carry transcribed spacers at this position in their 23S rRNA genes. At the 1850 region (E. coli numbering), Campylobacter 23S rRNA displayed a base pairing signature most like that of the beta and gamma subdivisions of the class Proteobacteria, but in the 270 region, Campylobacter 23S rRNA displayed a helix signature which distinguished it from the alpha, beta, and gamma subdivisions. Phylogenetic analysis comparing C. coli VC167 23S rRNA and a C. jejuni TGH9011 (ATCC 43431) 23S rRNA with 53 other completely sequenced (eu)bacterial 23S rRNAs showed that the two campylobacters form a sister group to the alpha, beta, and gamma proteobacterial 23S rRNAs, a positioning consistent with the idea that the genus Campylobacter belongs to the epsilon subdivision of the class Proteobacteria.
Publication date	1994-08
Publisher	American Society for Microbiology
Terms of use	Permission is granted to temporarily download one copy of the materials for personal, noncommercial transitory viewing only. NRC Publications Archive - Copyright and terms of use
Language	English
Peer reviewed	Yes
NPARC number	23002080
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Record identifier	3507dc8b-c14c-45f1-9cf7-902385e3394e
Record created	2017-08-08
Record modified	2019-03-11

Date modified:: 2019-03-21

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