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Uptake of exogenous DNA by plant protoplasts

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1139/b72-267
AuthorSearch for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. Prairie Regional Laboratory
FormatText, Article
Abstract
Uptake by plant protoplasts isolated from cultured cells was demonstrated using Escherichia coli¹⁴C-DNA and Ammi visnaga protoplasts. About 0.6 to 2.8% of exogenous E. coli¹⁴C-DNA was taken up into the protoplasts, of which about 20% appeared to be acid-precipitable. The time course of uptake, the effect of incubation temperature, and the effects of DNA and protoplast concentration were studied. DEAE-dextran, poly-L-lysine, and poly-L-ornithine were found to enhance the uptake markedly.
Publication date
PublisherCanadian Science Publishing
In
  • Canadian Journal of Botany 50, no. 10 (1 October 1972): 2077–2080.
LanguageEnglish
Peer reviewedYes
IdentifierNRCC-12772
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Record identifier36d2f23e-7638-4d6e-8a45-7302adeb0ef4
Record created2023-09-06
Record modified2023-09-06
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