| DOI | Resolve DOI: https://doi.org/10.1007/s11240-019-01704-5 |
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| Author | Search for: Wang, H. M.1; Search for: Enns, J. L.1; Search for: Nelson, K. L.1; Search for: Brost, J. M.1; Search for: Orr, T. D.1; Search for: Ferrie, A. M. R.1 |
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| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
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| Format | Text, Article |
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| Subject | doubled haploidy; histone deacetylase inhibitors; microspore culture; microspore embryogenesis; wheat |
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| Abstract | The objective of the study was to develop efficient widely adaptable microspore culture methodology for spring and winter wheat genotypes. Current wheat microspore culture methods are still inadequate for routine application, especially for winter genotypes. The present study involved increasing the frequency of microspore embryogenesis and improving the conversion of microspore-derived embryos to fertile green plants. A systematic evaluation was conducted testing numerous factors at all phases of the microspore embryogenesis process. Results from pretreatments, maltose gradients, and histone deacetylase inhibitors are presented. A 28 days cold pretreatment of spikes was optimal for winter wheat genotypes whereas a 21 days cold pretreatment was best for spring genotypes. Microspore suspensions containing a high frequency of embryogenic microspores were collected by resuspension in a 23% maltose gradient with subsequent pelleting with a mannitol containing solution. The application of trichostatin A (TSA) enhanced embryogenesis and/or green plant regeneration. Other epigenetic chemicals like scriptaid, BIX-01294, and sodium butyrate were also evaluated. Scriptaid enhanced embryogenesis but regenerated plantlets showed abnormal shoot and root development. The improved methodology was validated using a range of spring and winter wheat genotypes. |
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| Publication date | 2019-09-26 |
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| Publisher | Springer |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRC-ACRD-PDB018 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 377d2672-0ab6-4a76-8795-fdd5ae71c5db |
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| Record created | 2019-11-29 |
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| Record modified | 2020-03-16 |
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