| Author | Search for: Vilches, S.; Search for: Canals, R.; Search for: Wilhelms, M.; Search for: Salo, M.; Search for: Knirel, Y.; Search for: Vinogradov, Evguenii; Search for: Merino, S.; Search for: Tomas, J. |
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| Format | Text, Article |
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| Subject | acid; aeromonas; aeromonas hydrophila; band; biosynthesis; blood bactericidal activity; carbohydrate sequence; cell adhesion; cell line, tumor; chemistry; difference; DNA; DNA, bacterial; enzymology; fish diseases; galactose; gene; genetic complementation test; genetics; glucose; gram-negative bacterial infections; KDO; lethal dose 50; lipid; lipid A; lipopolysaccharide; LPS; mice; microbial viability; microbiology; molecular sequence data; mutant; mutation; O antigen; O1; pathogenicity; phosphate; physiology; residues; restricted; sepsis; sequence analysis, DNA; serotype; serum; strain; structure; synthesis; UTP-glucose-1-phosphate uridylyltransferase; veterinary; virulence |
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| Abstract | A mutation in galU that causes the lack of O34-antigen lipopolysaccharide (LPS) in Aeromonas hydrophila strain AH-3 was identified. It was proved that A. hydrophila GalU is a UDP-glucose pyrophosphorylase responsible for synthesis of UDP-glucose from glucose 1-phosphate and UTP. The galU mutant from this strain showed two types of LPS structures, represented by two bands on LPS gels. The first one (slow-migrating band in gels) corresponds to a rough strain having the complete core, with two significant differences: it lacks the terminal galactose residue from the LPS-core and 4-amino-4-deoxyarabinose residues from phosphate groups in lipid A. The second one (fast-migrating band in gels) corresponds to a deeply truncated structure with the LPS-core restricted to one 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) and three l-glycero-d-manno-heptose residues. galU mutants in several motile mesophilic Aeromonas strains from serotypes O1, O2, O11, O18, O21 and O44 were also devoid of the O-antigen LPS. The galU mutation reduced to less than 1 % the survival of these Aeromonas strains in serum, decreased the ability of these strains to adhere and reduced by 1.5 or 2 log units the virulence of Aeromonas serotype O34 strains in a septicaemia model in either fish or mice. All the changes observed in the galU mutants were rescued by the introduction of the corresponding single wild-type gene |
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| Publication date | 2007-08 |
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| In | |
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| Language | English |
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| NRC number | VILCHES2007 |
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| NPARC number | 9360808 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 3b47e912-a07d-4cd5-ac54-0066200f445d |
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| Record created | 2009-07-10 |
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| Record modified | 2020-05-10 |
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