Molecular cloning of an ester-forming triterpenoid: UDP-glucose 28-O-glucosyltransferase involved in saponin biosynthesis from the medicinal plant Centella asiatica

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DOI	Resolve DOI: https://doi.org/10.1016/j.plantsci.2017.05.009
Author	Search for: De Costa, Fernanda¹; Search for: Barber, Carla J. S.¹; Search for: Kim, Yeon-bok¹; Search for: Reed, Darwin W.¹; Search for: Zhang, Haixia¹; Search for: Fett-Neto, Arthur G.; Search for: Covello, Patrick S.¹
Name affiliation	National Research Council of Canada. Aquatic and Crop Resource Development
Format	Text, Article
Journal title	Plant Science
ISSN	0168-9452 1873-2259
Volume	262
Pages	9–17
Subject	centella asiatica; apiaceae; saponin biosynthesis; glucosyltransferase; UGT73AD1
Abstract	Triterpene saponins include bioactive compounds with structures consisting of triterpene aglycones (sapogenins) and one or more sugar moieties linked through acetal or ester glycosidic linkages at one or more sites. Centella asiatica (L.) Urban is a medicinal plant that contains bioactive ursane-type saponins, such as madecassoside and asiaticoside. In this work, glucosylation of triterpenoids in C. asiatica was investigated starting with plant extracts. An enzyme capable of glucosylating asiatic and madecassic acids was partially purified. Proteomics methods and cDNA sequence data were employed as tools to obtain a full-length cDNA clone encoding a glucosyltransferase. The recombinant gene product, UGT73AD1, was functionally expressed in Escherichia coli and purified by immobilized metal-affinity chromatography. Purified recombinant UGT73AD1 was found to have a narrow specificity, glucosylating asiatic and madecassic acids at the C28 carboxyl. mRNA accumulated in all tissues tested (leaves, stems, roots and flowers), with highest expression in leaves. Thus, UGT73AD1 was identified as a triterpenoid carboxylic acid: UDP-glucose 28-O-glucosyltransferase that appears to be involved in saponin biosynthesis in C. asiatica.
Publication date	2017-05-23
Publisher	Elsevier
Language	English
Peer reviewed	Yes
NRC number	NRC-ACRD-56094
NPARC number	23001956
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Record identifier	3f833732-aed7-4709-affa-c5c50fe1e171
Record created	2017-07-06
Record modified	2020-03-16

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Date modified:: 2020-07-07