| Abstract | We recently showed that Laser Ablation Electrospray Ionization (LAESI)-MS/MS could detect and quantify Domoic Acid (DA) directly from mussel tissue homogenates without sample extraction, cleanup or chromatographic separation [1]. The decrease in run time from ~ 20 min for LC methods to ~ 10 sec/sample for LAESI-MS is of interest to regulatory labs carrying out shellfish safety testing. Here, in collaboration with international regulatory partners, we assess the suitability of LAESI-MS as a high-throughput screening or quantitation tool for DA in a variety of shellfish matrices. The method was first optimized for use with high resolution MS detection. Samples analyzed included 190 shellfish samples previously analyzed by regulatory labs and DA certified reference materials. LAESI-MS shows promise as a screening tool capable of differentiating samples above and below 5 mg/kg, compatible with the action level of 20 mg/kg set for DA in edible shellfish tissue. 1. DG Beach, CM Walsh, P McCarron. Toxicon 2014, 92, 75-80. |
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