| Abstract | Plant cell and tissue culture has served as methodology of physiology and biochemistry in a quest to increase our knowledge of cell biology for half a century. Today, tissue culture has largely been integrated in biotechnology and permits the regeneration of plants as clones and as transgenics. Callus and cell suspension cultures, the pride of laboratories for years, are being phased out; cell phenomena are being studied in planta by employing molecular probes. Also, cells cultured in vitro carry a factor of uncertainty in somaclonal variation. Callus and cell suspensions, thus, become reduced in importance to source material for certain enzymes, chemicals, and experimental material for special aspects of differentiation. Changes in goals require changes in treatment of methodology of plant tissue culture. |
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