DOI | Resolve DOI: https://doi.org/10.1021/ja312665r |
---|
Author | Search for: Marquardt, D.; Search for: Williams, J.A.; Search for: Kučerka, N.1; Search for: Atkinson, J.; Search for: Wassall, S.R.; Search for: Katsaras, J.1; Search for: Harroun, T.A. |
---|
Affiliation | - National Research Council of Canada. NRC Canadian Neutron Beam Centre
|
---|
Format | Text, Article |
---|
Subject | Antioxidant mechanisms; Hydrocarbon matrix; Hydrophobic-hydrophilic; Lipid acyl-chains; Measurements of; Oxidation kinetics; Physical locations; Reactive oxygen species; Cytology; Lipid bilayers; Neutron diffraction; Ultraviolet spectroscopy; Physical addresses; alpha tocopherol; hydrocarbon; hydrogen; hydroxyl group; reactive oxygen metabolite; tocopherol; antioxidant activity; hydration; hydrophilicity; hydrophobicity; lipid bilayer; lipid membrane; lipid oxidation; membrane structure; neutron diffraction; nuclear magnetic resonance spectroscopy; review; ultraviolet spectroscopy |
---|
Abstract | We show evidence of an antioxidant mechanism for vitamin E which correlates strongly with its physical location in a model lipid bilayer. These data address the overlooked problem of the physical distance between the vitamin's reducing hydrogen and lipid acyl chain radicals. Our combined data from neutron diffraction, NMR, and UV spectroscopy experiments all suggest that reduction of reactive oxygen species and lipid radicals occurs specifically at the membrane's hydrophobic-hydrophilic interface. The latter is possible when the acyl chain "snorkels" to the interface from the hydrocarbon matrix. Moreover, not all model lipids are equal in this regard, as indicated by the small differences in vitamin's location. The present result is a clear example of the importance of lipid diversity in controlling the dynamic structural properties of biological membranes. Importantly, our results suggest that measurements of aToc oxidation kinetics, and its products, should be revisited by taking into consideration the physical properties of the membrane in which the vitamin resides. © 2013 American Chemical Society. |
---|
Publication date | 2013 |
---|
In | |
---|
Language | English |
---|
Peer reviewed | Yes |
---|
NPARC number | 21269847 |
---|
Export citation | Export as RIS |
---|
Report a correction | Report a correction (opens in a new tab) |
---|
Record identifier | 439a0a03-56d5-4ac9-981f-7ec9500f26a3 |
---|
Record created | 2013-12-13 |
---|
Record modified | 2020-04-22 |
---|