| DOI | Resolve DOI: https://doi.org/10.1039/c3ay40652k |
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| Author | Search for: Drake, P.; Search for: Jiang, P.-S.; Search for: Chang, H.-W.; Search for: Su, S.-C.; Search for: Tanha, J.1; Search for: Tay, L.-L.1; Search for: Chen, P.; Search for: Lin, Y.-J. |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Subject | 4-mercaptobenzoic acids; Coated nanoparticles; Detection and quantifications; Gold Nanoparticles; Pathogenic bacterium; Single-domain antibodies; Staphylococcus aureus; Surface enhanced Raman spectroscopy; Antibodies; Bacteria; Chemical detection; Gold; Iron oxides; Metal nanoparticles; Raman spectroscopy; Transmission electron microscopy; Nanomagnetics |
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| Abstract | Nanoparticle labels were used to detect the pathogenic bacteria Staphylococcus aureus. Two different labels were employed. These consisted of nanoparticles based on iron-oxide for magnetic trapping and isolation of the bacteria and surface enhanced Raman spectroscopy (SERS) active gold nanoparticles (AuNPs) coated with 4-mercaptobenzoic acid for the detection and quantification of the bacteria. Both types of nanoparticles were conjugated with single domain antibodies that selectively bind to protein A on the surface of S. aureus. Transmission electron microscopy (TEM) image analysis showed the iron-oxide nanoparticles to have magnetic core diameters of 20 nm ± 3 nm and the gold nanoparticles to be 51 nm ± 2 nm in diameter. A response curve was obtained displaying a logarithmic dependency between the 1073 cm -1 peak intensity in the SERS spectra of the AuNPs and the concentration of S. aureus cells in the initial sample. The overall detection limit was estimated to be 1 S. aureus cell mL-1 in less than 10 min without culturing. © 2013 The Royal Society of Chemistry. |
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| Publication date | 2013 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21270950 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 44f8758e-881a-464b-9268-280a78a6cbf4 |
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| Record created | 2014-02-18 |
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| Record modified | 2020-04-22 |
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