Preparation and certification of solutions of perdeuterated polycyclic aromatic compounds intended for use as surrogate internal standards

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1007/BF00323170
Author	Search for: Quilliam, Michael A.¹ ; Search for: Hardstaff, William R.¹ ; Search for: Anacleto, Joseph F.¹ ; Search for: Leblanc, M. Denise¹ ; Search for: Stergiopoulos, Victonia¹ ; Search for: Dick, Karla L.¹ ; Search for: Bowser, Michael T.¹ ; Search for: Curtis, Jonathan M.¹ ; Search for: Embree, Donald J.¹ ; Search for: Sim, P. Greig¹ ; Search for: Boyd, Robert K.¹
Name affiliation	National Research Council Canada. NRC Institute for Marine Biosciences
Format	Text
Type	Article
Journal title	Fresenius' Journal of Analytical Chemistry
ISSN	0937-0633 1618-2650
Volume	350
Issue	3
Pages	109–118
Abstract	Two standard solutions of deuterated polycyclic aromatic compounds (PACs) have been prepared for use as surrogate internal standards. Solution DPAC-1 contains 21 deuterated PACs, and is intended for use with mass spectrometric (MS) detection. Most of the difficulties in certifying concentrations in DPAC-1 arose from the fact that none of the individual compounds was 100% deuterated, so that effects of mass spectrometric fragmentation are convoluted with those of isotopic distributions. The best methods are discussed for using such internal standards so as to minimize these problems, together with those arising from kinetic isotope effects. Solution DPAC-2 contains 6 deuterated PACs, and is primarily intended for use with reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection (FLD, dual programmed wavelength mode), in which the signals for analyte and internal standard are separated chromatographically rather than via the detector. Full details of the preparation of these solutions are described. In addition, examples of their use in the analysis of a certified coal-tar extract (NIST SRM 1597) are described briefly. In one example a novel HPLC-MS technique was employed, and in the other the HPLC-FLD technique was used.
Publication date	1994
Terms of use	Permission is granted to temporarily download one copy of the materials for personal, noncommercial transitory viewing only. NRC Publications Archive - Copyright and terms of use
Language	English
Peer reviewed	Yes
NRC number	38030
NPARC number	23000899
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Record identifier	45417d65-3cd2-4d46-8d50-3c05f3409f8d
Record created	2016-11-07
Record modified	2016-11-07

Date modified:: 2019-03-26

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