The relative contributions of vitamin E, urate, ascorbate and proteins to the total peroxyl radical-trapping antioxidant activity of human blood plasma

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/0304-4165(87)90155-3
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Name affiliation
  1. National Research Council of Canada
FormatText, Article
Journal titleBBA - General Subjects
ISSN0304-4165
Volume924
Issue3
Pages408–419
Subjectalpha tocopherol; antioxidant; ascorbic acid; free radical; lipid peroxide; perhydroxyl radical; peroxide; plasma protein; uric acid; biosynthesis; blood; physiology; solubility; Antioxidants; Ascorbic Acid; Blood Proteins; Free Radicals; Lipid Peroxides; Peroxides; Solubility; Support, Non-U.S. Gov't; Uric Acid; Vitamin E
Abstract
The Total (Peroxyl) Radical-trapping Antioxidant Parameter (TRAP) of six freshly prepared human plasma samples and 45 frozen plasma samples has been determined. It is shown that contributions from urate (35-65%), plasma proteins (10-50%), ascorbate (0-24%) and vitamin E (5-10%) to TRAP account for all of the peroxyl radical-trapping antioxidant activity in the majority of the samples. The changes in concentrations of the plasma antioxidants during peroxyl radical attack show that first line of defense is provided by the plasma sulfhydryl group, even urate being spared during the initial stages of the reaction. The modes of action of all of these plasma antioxidants and possible interactions between them are discussed, with particular emphasis on the abilities of the water-soluble antioxidants to regenerate or spare the only lipid-soluble antioxidant, vitamin E. © 1987.
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LanguageEnglish
Peer reviewedYes
NPARC number21276506
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