| DOI | Resolve DOI: https://doi.org/10.1002/btpr.2761 |
|---|
| Author | Search for: Karakach, Tobias K.1; Search for: Dachon, Aurore2; Search for: Choi, Jerome3; Search for: Miguez, Carlos4; Search for: Masson, Luke2; Search for: Tartakovsky, Boris3 |
|---|
| Affiliation | - National Research Council of Canada. Measurement Science and Standards
- National Research Council of Canada. Human Health Therapeutics
- National Research Council of Canada. Energy, Mining and Environment
- National Research Council of Canada. Aquatic and Crop Resource Development
|
|---|
| Format | Text, Article |
|---|
| Subject | multivariate curve resolution; fluorescence; on‐line diagnostics; monitoring; bioreactor |
|---|
| Abstract | This study describes the application of the multivariate curve resolution (MCR) analysis technique for real‐time analysis of culture fluorescence during recombinant Pichia pastoris cultivation in a bioreactor. Fluorescence spectra were acquired with an on‐line dual excitation wavelength fluorometer and then used to develop a real time MCR‐based bioprocess monitoring and diagnostics tool. Initial bioreactor experiments using two similar recombinant antibody secreting P. pastoris cell lines showed significant differences in protein production. To distinguish between the contributions of operating conditions and the specific cell line's genetic composition to the observed differences in protein production, the bioreactor experiments were repeated and accompanied by real time MCR analysis. The tests demonstrated high sensitivity of MCR‐derived “pure concentration” profiles to growth as well as to initial conditions, thus enabling real‐time cultivation process trend diagnostics and fault detection. |
|---|
| Publication date | 2018-12-02 |
|---|
| Publisher | Wiley |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 466e4ecb-8a9f-4b0b-a713-42ad1e5564d9 |
|---|
| Record created | 2019-04-12 |
|---|
| Record modified | 2020-03-16 |
|---|
