| DOI | Resolve DOI: https://doi.org/10.1039/c0an00897d |
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| Author | Search for: Tay, L.-L.1; Search for: Tremblay, R.G.2; Search for: Hulse, J.1; Search for: Zurakowski, B.2; Search for: Thompson, M.; Search for: Bani-Yaghoub, M.2 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Microstructural Sciences
- National Research Council of Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | amide; caspase 3; brain injury; chemistry; discriminant analysis; enzymology; metabolism; methodology; mouse; pathology; principal component analysis; Raman spectrometry; Amides; Brain Injuries; Caspase 3; Discriminant Analysis; Mice; Principal Component Analysis; Spectrum Analysis, Raman |
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| Abstract | Brain injury can lead to irreversible tissue loss and functional deficit along with significant health care costs. Raman spectroscopy can be used as a non-invasive technique to provide detailed information on the molecular composition of diseased and damaged tissues. This technique was used to examine acute mouse brain injury, focusing on the motor cortex, a region directly involved in controlling execution of movement. The spectral profile obtained from the injured brain tissue revealed a markedly different signature, particularly in the amide I and amide III vibrational region when compared to that of healthy brain tissue. Most noticeably, there was a significant reduction of the amide I vibration at the acute injury site and the appearance of two distinct features at 1586 and 1618 cm -1. Complementary immunohistochemical analysis of the injured brain tissue showed an abundant expression of Caspase 3 (a cysteine protease marker used for apoptosis), suggesting that the injury-induced specific Raman shifts may be correlated with cell death. Taken together, this study demonstrates that Raman spectroscopy can play an important role in detecting the changes that occur in the injured brain and provide a possible technology for monitoring the recovery process. © 2011 The Royal Society of Chemistry. |
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| Publication date | 2011 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21271039 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 4769f3fa-1dd9-46a1-902d-2de53ab0ce8d |
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| Record created | 2014-03-24 |
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| Record modified | 2020-04-21 |
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