| Abstract | Resonance Raman spectra of cinnamoyl and α-toluyl acyl enzymes of α-chymotrypsin have been obtained. Bands associated with the aromatic portion of the acylating groups were identified and could be distinguished in a cinnamoyl derivative from those associated with the ethylenic residue. Spectral differences in the acyl enzyme relative to substrate and product were observed. These differences, which represent changes in vibrational modes of substrate bonds due to specific interaction with the active site, provide a novel approach to the study of the catalytic mechanisms of enzymes. |
|---|
